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Provedor de dados:  BJM
País:  Brazil
Título:  Laccase produced by a thermotolerant strain of Trametes trogii LK13
Autores:  Yan,Jinping
Chen,Yuhui
Niu,Jiezhen
Chen,Daidi
Chagan,Irbis
Data:  2015-03-01
Ano:  2015
Palavras-chave:  Trametes trogii
Thermotolerant fungi
Laccase
Thermostability
Organic cosolvent tolerance
Resumo:  Thermophilic and thermotolerant micro-organisms strains have served as the natural source of industrially relevant and thermostable enzymes. Although some strains of the Trametes genus are thermotolerant, few Trametes strains were studied at the temperature above 30 °C until now. In this paper, the laccase activity and the mycelial growth rate for Trametes trogii LK13 are superior at 37 °C. Thermostability and organic cosolvent tolerance assays of the laccase produced at 37 °C indicated that the enzyme possessed fair thermostability with 50% of its initial activity at 80 °C for 5 min, and could remain 50% enzyme activity treated with organic cosolvent at the concentration range of 25%–50% (v/v). Furthermore, the test on production of laccase and lignocellulolytic enzymes showed the crude enzymes possessed high laccase level (1000 U g−1) along with low cellulose (2 U g−1) and xylanase (140 U g−1) activity. Thus, T. trogii LK13 is a potential strain to be applied in many biotechnological processes.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100059
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246120130895
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.1 2015
Direitos:  info:eu-repo/semantics/openAccess
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